UV-Vis Spectrophotometer and Beer-Lambert Law

Objectives

• .To perform dilutions

• To use a spectrophotometer

• To distinguish the wavelength of minimum %T for a solution

• To relate light transmission with the concentration of a solution

• To formulate a way to find the concentration of blue dye in a sports drink

• To identify origins of experimental error.

Curriculum alignments

• NGSS

HS-PS4-4: Evaluate the validity and reliability of claims in published materials of the effects that different frequencies of electromagnetic radiation have when absorbed by matter.

• AP

3.11 Spectroscopy and the Electromagnetic Spectrum

• Categories: Chemistry Laboratory