Quantitative Protein Assay Using Biuret Reagent

• To state that amino acids react with each other to form proteins and form peptide bonds during this reaction

• To test the presence or absence of proteins qualitatively with the help of Biuret reagent

• To prepare standard solutions using a protein stock solution

• To acquire the absorbance values of the prepared standard solutions using Biuret reagent and to plot a calibration curve

• To calculate the concentration of protein solution with an unknown concentration by using the equation of the calibration curve

LS1-6 From Molecules to Organisms: Structures and Processes

2.1 Testing for biological molecules

2.1 Molecules to metabolism