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Quantitative Protein Assay Using Biuret Reagent



• To state that amino acids react with each other to form proteins and form peptide bonds during this reaction

• To test the presence or absence of proteins qualitatively with the help of Biuret reagent

 • To prepare standard solutions using a protein stock solution

• To acquire the absorbance values of the prepared standard solutions using Biuret reagent and to plot a calibration curve

• To calculate the concentration of protein solution with an unknown concentration by using the equation of the calibration curve


Curriculum alignments


LS1-6 From Molecules to Organisms: Structures and Processes


• Cambridge AS & A Level

2.1 Testing for biological molecules 



2.1 Molecules to metabolism



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