Objectives
• To state that amino acids react with each other to form proteins and form peptide bonds during this reaction
• To test the presence or absence of proteins qualitatively with the help of Biuret reagent
• To prepare standard solutions using a protein stock solution
• To acquire the absorbance values of the prepared standard solutions using Biuret reagent and to plot a calibration curve
• To calculate the concentration of protein solution with an unknown concentration by using the equation of the calibration curve
Curriculum alignments
• NGSS
LS1-6 From Molecules to Organisms: Structures and Processes
• Cambridge AS & A Level
2.1 Testing for biological molecules
• IB DP
2.1 Molecules to metabolism